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The high resolution, proper refinement strategy and careful modeling allowed the positioning of a large part of the hydrogen atoms in the structure. The Ni-H – and Fe-H – bond lengths were 1. Furthermore, we could assign the Fe-CO and CN – ligands at the active site, and could obtain the H-bond networks and the preferred proton transfer pathway in the hydrogenase.

The small and large subunits are shown in green and gray, respectively. Schematic representation of the hydrogen, proton and electron pathways is shown with the red arrows. The blue and green meshes represent the 2Fo-Fc and Fo-Fc electron density maps, respectively. Structure of the month – July Nature

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